Mannosylated Polyethyleneimine–Hyaluronan Nanohybrids for Targeted Gene Delivery to Macrophage-Like Cell Lines
نویسندگان
چکیده
منابع مشابه
Mannosylated polyethyleneimine-hyaluronan nanohybrids for targeted gene delivery to macrophage-like cell lines.
Nonviral gene delivery systems have a number of limitations including low transfection efficiency, specificity, and cytotoxicity, especially when the target cells are macrophages. To address these issues, the hypothesis tested in this study was that mannose functionalized nanohybrids composed of synthetic and natural polymers will improve transfection efficiency, cell viability, and cell specif...
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BACKGROUND Liposomes can be modified with different ligands to control their biological properties, such as longevity, targeting ability, and intracellular penetration, in a desired fashion. The aim of this study was to modify liposomes with a novel mannosylated polyethylene glycol-phosphatidylethanolamine (M-PEG-PE) ligand to achieve active targeted gene delivery. METHODS Rat Kupffer cells w...
متن کاملMannosylated biodegradable polyethyleneimine for targeted DNA delivery to dendritic cells
BACKGROUND To establish a potential gene-delivery system with the ability to deliver plasmid DNA to dendritic cells (DCs) more efficiently and specifically, we designed and synthesized a low-molecular-weight polyethyleneimine and triethyleneglycol polymer (PEI-TEG) and a series of its mannosylated derivatives. METHODS PEI-TEG was synthesized from PEI2000 and PEI600 with TEG as the cross-linke...
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Gaucher disease (GD) is a rare monogenetic disorder leading to dysfunction of acid β-glucosidase (β-glucocerebrosidase; GCase) and accumulation of glucosylceramide in lysosomes, especially in macrophages (Gaucher cells). Many of the mutations at the origin of GD do not impair the catalytic activity of GCase, but cause misfolding and subsequent degradation by the quality control system at the en...
متن کاملMannosylated Chitosan Nanoparticles for Delivery of Antisense Oligonucleotides for Macrophage Targeting
The therapeutic potential of antisense oligonucleotides (ASODN) is primarily dependent upon its safe and efficient delivery to specific cells overcoming degradation and maximizing cellular uptake in vivo. The present study focuses on designing mannosylated low molecular weight (LMW) chitosan nanoconstructs for safe ODNs delivery by macrophage targeting. Mannose groups were coupled with LMW chit...
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ژورنال
عنوان ژورنال: Bioconjugate Chemistry
سال: 2012
ISSN: 1043-1802,1520-4812
DOI: 10.1021/bc200599k